Trackability of proteins from probiotic Bifidobacterium spp. in the gut using metaproteomics

Abstract Beneficial effects of Bifidobacterium spp. on gut microbiota development and infant health have been reported earlier. Therefore, supplementation formulas with probiotic bifidobacteria, as well prebiotics stimulating bifidobacterial growth, has proposed. Here, we studied the faecal microbiome infants supplemented specialized nutrition, which some received a standard amino acid-based formula (AAF) others an AAF specific mixture (synbiotics) using metaproteomics 16S rRNA gene sequencing. Faecal samples were taken at baseline, after 6 12 months fed formula. The aim was to compare microbial differences between treated those who additional synbiotics. Our findings show that synbiotics significantly higher levels Bifidobacteriaceae DNA increased Coriobacteriaceae proteins, over time. Moreover, visit carbohydrate-active enzymes, known metabolize oligosaccharides, observed in synbiotic group compared non-synbiotic group. results indicate provided our study are trackable protein level during intervention period.